Extracellular Matrix Mimetic Peptide Scaffolds for Neural Stem Cell Culture and Differentiation

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1202)

Abstract

Self-assembled peptide nanofibers form three-dimensional networks that are quite similar to fibrous extracellular matrix (ECM) in their physical structure. By incorporating short peptide sequences derived from ECM proteins, these nanofibers provide bioactive platforms for cell culture studies. This protocol provides information about preparation and characterization of self-assembled peptide nanofiber scaffolds, culturing of neural stem cells (NSCs) on these scaffolds, and analysis of cell behavior. As cell behavior analyses, viability and proliferation of NSCs as well as investigation of differentiation by immunocytochemistry, qRT-PCR, western blot, and morphological analysis on ECM mimetic peptide nanofiber scaffolds are described.

Keywords

Peptide nanofibers Scaffolds Self-assembly Hydrogels Neural stem cells 

Notes

Acknowledgments

B.M. is supported by Scientific and Technological Research Council of Turkey (TUBITAK) grant number 111M410. M.O.G and A.B..T. acknowledge support from the Turkish Academy of Sciences Distinguished Young Scientist Award (TUBA-GEBIP).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent UniversityAnkaraTurkey

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