Abstract
Due to the increasing use of engineered nanoparticles in many consumer products, rapid and economic tests for evaluating possible adverse effects on human health are urgently needed. In the present chapter the use of mouse embryonic stem cells as a valuable tool to in vitro screen nanoparticle toxicity on embryonic tissues is described. This in vitro method is a modification of the embryonic stem cell test, which has been widely used to screen soluble chemical compounds for their embryotoxic potential. The test offers an alternative to animal experimentation, reducing experimental costs and ethical issues.
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Visualization of contracting EBs at the end of the differentiation experiments (AVI 15,590 kb)
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Campagnolo, L., Fenoglio, I., Massimiani, M., Magrini, A., Pietroiusti, A. (2013). Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 1058. Humana Press, Totowa, NJ. https://doi.org/10.1007/7651_2013_11
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DOI: https://doi.org/10.1007/7651_2013_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-570-5
Online ISBN: 978-1-62703-571-2
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