Extraction of Total RNA from Yersinia pestis
Bacterial RNA abundance is monitored to analyze gene expression at the transcriptional level. RNA is typically extracted using organic solvents or commercially available kits. Appropriate techniques for total RNA extraction should be used to harvest sufficient starting material required for further analysis while taking care not to bias sampling of the transcriptome. Here, we describe two methods to obtain high-quality RNA from Yersinia pestis by using TRIzol® Reagent and the PureLink™ RNA Mini Kit. The first method allows us to disrupt and dissolve bacterial cells by using TRIzol® Reagent, followed by phase separation and RNA precipitation. The alternative method using the PureLink™ RNA Mini Kit contains a column purification step. After homogenization, the sample is processed through a membrane to purify RNA.
Key wordsRNA Extraction Yersinia pestis TRIzol Reagent RNA Purification