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Inducible Gene Expression in Yersinia pestis

  • Juping Zhao
  • Yicheng Sun
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Inducible gene expression systems are usually used to characterize gene functions. Many promoter systems have been used to induce gene expression, but only a few of them are commonly used. Here, we introduce the PBAD promoter system, which can be used for the tight regulation of gene expression in Yersinia pestis. The PBAD promoter system allows high-level, tightly regulated protein expression. When a gene is cloned downstream from the PBAD promoter, its expression levels can be regulated by varying the concentration of l-arabinose. The tight regulation of PBAD by AraC is especially useful for the expression of potentially toxic or essential genes.

Key words

Yersinia pestis Gene expression PBAD 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.MOH Key Laboratory of Systems Biology of PathogensInstitute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

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