Inducible gene expression systems are usually used to characterize gene functions. Many promoter systems have been used to induce gene expression, but only a few of them are commonly used. Here, we introduce the PBAD promoter system, which can be used for the tight regulation of gene expression in Yersinia pestis. The PBAD promoter system allows high-level, tightly regulated protein expression. When a gene is cloned downstream from the PBAD promoter, its expression levels can be regulated by varying the concentration of l-arabinose. The tight regulation of PBAD by AraC is especially useful for the expression of potentially toxic or essential genes.
Yersinia pestisGene expression PBAD
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Terpe K (2006) Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 72(2):211–222CrossRefPubMedGoogle Scholar
Newman JR, Fuqua C (1999) Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator. Gene 227(2):197–203CrossRefPubMedPubMedCentralGoogle Scholar
Khlebnikov A, Skaug T, Keasling JD (2002) Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol 29(1):34–37CrossRefPubMedGoogle Scholar
Haldimann A, Daniels LL, Wanner BL (1998) Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon. J Bacteriol 180(5):1277–1286PubMedPubMedCentralGoogle Scholar
Carson MJ, Barondess J, Beckwith J (1991) The FtsQ protein of Escherichia coli: membrane topology, abundance, and cell division phenotypes due to overproduction and insertion mutations. J Bacteriol 173(7):2187–2195CrossRefPubMedPubMedCentralGoogle Scholar
Dalbey RE, Wickner W (1985) Leader peptidase catalyzes the release of exported proteins from the outer surface of the Escherichia coli plasma membrane. J Biol Chem 260(29):15925–15931PubMedGoogle Scholar
Guzman LM, Barondess JJ, Beckwith J (1992) FtsL, an essential cytoplasmic membrane protein involved in cell division in Escherichia coli. J Bacteriol 174(23):7716–7728CrossRefPubMedGoogle Scholar
Kuhn A, Wickner W (1985) Isolation of mutants in M13 coat protein that affect its synthesis, processing, and assembly into phage. J Biol Chem 260(29):15907–15913PubMedGoogle Scholar
Russell CB, Stewart RC, Dahlquist FW (1989) Control of transducer methylation levels in Escherichia coli: investigation of components essential for modulation of methylation and demethylation reactions. J Bacteriol 171(7):3609–3618CrossRefPubMedPubMedCentralGoogle Scholar
San Millan JL, Boyd D, Dalbey R, Wickner W, Beckwith J (1989) Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase. J Bacteriol 171(10):5536–5541CrossRefPubMedPubMedCentralGoogle Scholar