Patch Clamp Techniques pp 137-145 | Cite as
Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms
Protocol
Abstract
The synapse plays pivotal roles in various neuronal functions, with its efficacy determining the opening and closing of neuronal circuits. Among the parameters that determine synaptic efficacy, presynaptic factors are of crucial importance. However, these factors are among those that are the least well studied. Patch-clamp recording from the calyx of Held presynaptic terminal, described in this chapter, has enabled us to address directly questions about the presynaptic regulatory mechanisms in synaptic transmission.
Keywords
Presynaptic Terminal Membrane Capacitance Patch Pipette Access Resistance Personal Computer Monitor
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Notes
Acknowledgments
We thank Ervin Johnson for helpful comments and English-language editing.
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