Interphase FISH for Detection of Chromosomal Mosaicism

  • Ivan Y. Iourov
  • Svetlana G. Vorsanova
  • Yuri B. Yurov
Part of the Springer Protocols Handbooks book series (SPH)


Interphase fluorescence in situ hybridization (iFISH) allows qualitative and quantitative detection of chromosomal DNA at all stages of the cell cycle and at molecular resolutions. Accordingly, this methodology offers an opportunity to address chromosome numbers and structures in all the human tissues. In this light, a variety of iFISH techniques have been acknowledged as indispensable for studying intercellular genomic variation or somatic chromosomal mosaicism. Here, an interphase FISH protocol for the detection of intercellular genomic variations and low-level chromosomal mosaicism in somatic tissues is described. Additionally, technical issues influencing the results of iFISH are considered.


Interphase fluorescence in situ hybridization (iFISH) Evaluation Mosaicism Molecular resolution 



The chapter is dedicated to Ilia V. Soloviev. The authors are supported by a grant from the Russian Science Foundation (project #14-35-00060).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Ivan Y. Iourov
    • 1
    • 2
    • 3
  • Svetlana G. Vorsanova
    • 1
    • 2
    • 4
  • Yuri B. Yurov
    • 1
    • 2
    • 4
  1. 1.Mental Health Research CenterMoscowRussia
  2. 2.Russian National Research Medical University named after N. I. Pirogov, Separated Structural Unit “Clinical Research Institute of Pediatrics”, Ministry of Health of Russian FederationMoscowRussia
  3. 3.Department of Medical GeneticsRussian Medical Academy of Postgraduate EducationMoscowRussia
  4. 4.Moscow State University of Psychology and EducationMoscowRussia

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