cenM-FISH Approaches

Protocol
First Online:
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Multicolor-FISH using whole-chromosome painting and FISH banding with partial-chromosome painting probes are for technical reasons not suited to characterize small centromeric and pericentromeric rearrangements. Thus, probe sets to characterize the different human centromeres, and others to enable studies of the centromere-near regions of human chromosomes were established. Such centromere-specific multicolor (cenM)- and subcentromere-specific (subcenM)-FISH techniques are presented in this chapter. How to set up and use these probe sets is reported.

Keywords

Centromere Centromere-specific multicolor-FISH (cenM-FISH) Subcentromere-specific multicolor-FISH (subcenM-FISH) Centromere-near imbalances Small supernumerary marker chromosomes (sSMCs) 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Jena University Hospital, Friedrich Schiller University, Institute of Human GeneticsJenaGermany

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