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scFv by Two-Step Cloning

  • Dafne MüllerEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The scFv-chain Fv (scFv) corresponds to the smallest functional binding unit of a classical antibody and constitutes a favourite format for the comparative first evaluation of the recombinant potential of an antibody. In this protocol, a two-step cloning strategy is described following the modular exchange principle as a general guideline for converting antibodies into an scFv format suitable for periplasmatic expression in E. coli.

Keywords

Calf Intestine Alkaline Phosphatase Periplasmatic Expression pelB Leader Antibody Fusion Protein Respective Restriction Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Institute of Cell Biology and ImmunologyUniversity of StuttgartStuttgartGermany

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