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Xenograft Mouse Models for Tumour Targeting

  • Surinder K. SharmaEmail author
  • R Barbara Pedley
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Monoclonal antibodies against tumour-associated antigens have been widely used for targetted therapy of cancer. Most of the pre-clinical assessment of efficacy and toxicity of these agents is carried out in immunodeficient mice xenografted with human tumours. Quantitative biodistribution is routinely assessed by administration of radiolabelled antibodies, followed by counting of radioactivity in tumour and normal tissues. However, in order to optimise the therapy design and synergistic combination of agents, it is desirable to understand the complex antibody-tumour interactions in-vivo and determine which regions of tumours are being targetted. This is achieved by quantitative, high resolution fluorescence microscopy, which can be employed to demonstrate the distribution and therapeutic efficacy of the targetting antibody in relation to the tumour microenvironment.

Keywords

LS174T Cell PD10 Column Welwyn Garden City Green Needle Radiolabelled Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Dr Ethaar El-Emir for skillful technical assistance and Cancer Research UK and European Union FP7 (201342-ADAMANT) for grant support.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.UCL Cancer InstituteUniversity College LondonLondonUK

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