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Standardized Sequence and Structure Analysis of Antibody Using IMGT®

  • François Ehrenmann
  • Patrice Duroux
  • Véronique Giudicelli
  • Marie-Paule LefrancEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Standardized sequence and structure analysis of antibody using IMGT®, the international ImMunoGeneTics information system® (http://www.imgt.org), allows to bridge, for the first time, the gap between antibody sequences and three-dimensional (3D) structures. This is achieved through the IMGT Scientific chart rules, based on IMGT-ONTOLOGY. IMGT® is the international reference for immunogenetics and immunoinformatics, and its standards (IMGT nomenclature and labels and IMGT unique numbering) are particularly useful for antibody humanization and evaluation of immunogenicity. In IMGT®, the antibody analysis from nucleotide sequence to 2D structure is supported by the widely used IMGT/V-QUEST system and the integrated IMGT/JunctionAnalysis software. The IMGT® approach from amino acid sequence to 3D structure is supported by the IMGT/DomainDisplay, IMGT/DomainGapAlign and IMGT/Collier-de-Perles tools, by some important features of IMGT/2Dstructure-DB, for antibodies for which 3D structures are not yet available (INN entries) and IMGT/3Dstructure-DB and associated tools, for crystallized antibodies (Contact analysis, IMGT/StructuralQuery, IMGT/DomainSuperimpose).

Keywords

Versus Domain Contact Analysis Synthesis View Result Display International Nonproprietary Name 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Chantal Ginestoux for the figures. We are very grateful to Gérard Lefranc for helpful discussions and to the IMGT® team for its expertise and constant motivation. IMGT® is a registered trademark of CNRS. IMGT® is member of the International Medical Informatics Association (IMIA). IMGT® was funded in part by the BIOMED1 (BIOCT930038), Biotechnology BIOTECH2 (BIO4CT960037) and 5th PCRDT Quality of Life and Management of Living Resources programmes (QLG2-2000-01287) programmes of the European Union (EU). IMGT® is currently supported by the Centre National de la Recherche Scientifique (CNRS), the Ministère de l'Enseignement Supérieur et de la Recherche (MESR) (Université Montpellier 2 Plan Pluri-Formation, Institut Universitaire de France), the GIS IBiSA, the Région Languedoc-Roussillon (Grand Plateau Technique pour la Recherche Région (GPTR) Languedoc-Roussillon), the Agence Nationale de la recherche ANR (BIOSYS06_135457, FLAVORES) and the EU 6th PCRDT ImmunoGrid (FP6 IST-028069).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • François Ehrenmann
    • 1
  • Patrice Duroux
    • 1
  • Véronique Giudicelli
    • 1
  • Marie-Paule Lefranc
    • 1
    Email author
  1. 1.IMGT®, the international ImMunoGeneTics Information System®, Laboratoire d’ImmunoGénétique Moléculaire LIGMUniversité Montpellier 2, Institut de Génétique HumaineMontpellier Cedex 5France

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