Fusion Proteins with Improved PK
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Small recombinant antibody formats are rapidly cleared from circulation. The coupling of these molecules to human serum albumin (HSA) is an efficient strategy to prolong their in vivo half-life. Two methods are described in this chapter for the attachment of HSA to small antibody molecules: the direct fusion of HSA and the indirect coupling by the fusion of an albumin-binding domain to the antibody. Furthermore, an assay is described to verify the functionality of the albumin-binding domain, and a method is proposed to determine the antibodies serum half-life in mice.
KeywordsFusion Protein Human Serum Albumin HEK293 Cell Immobilize Metal Affinity Chromatography Small Antibody
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