Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay

  • Tina OtzEmail author
Part of the Springer Protocols Handbooks book series (SPH)

Monoclonal antibodies (mAbs) can exert their biological functions by a large variety of mechanisms and have therefore become a major part in the treatment of diseases like viral infections and cancer. For the clinical efficacy of many mAbs Fc-mediated effector functions like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) play an essential role. This protocol describes a simple method to test whether a monoclonal antibody is able to induce ADCC or CDC using a chromium release assay.


Natural Killer Cell Target Cell Effector Cell Buffy Coat Antigen Density 
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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.University of StuttgartInstitute for Cell Biology and ImmunologyStuttgartGermany

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