Generation of Heavy and Light Chains (Chimeric Antibodies)

  • Kirstin A. ZettlitzEmail author
Part of the Springer Protocols Handbooks book series (SPH)

Antibodies are highly specific, naturally evolved molecules that recognize and eliminate pathogens and tumour associated antigens. The organisation into distinct structural and functional domains facilitates their engineering. Driven by a variety of antibody engineering methods and technological developments, chimeric and humanized antibodies are commonly used as therapeutics for cancer, infectious and inflammatory diseases, for imaging or in targeted delivery systems. This chapter describes the cloning and expression of chimeric antibodies using Lonza’s “double-gene vectors” (Lonza Biologics’ Glutamine Synthetase (GS) gene expression system) in mammalian cells.


Glutamine Synthetase Chimeric Antibody Light Chain Gene Light Chain Variable Region Constant Region Gene 
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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Institute of Cell Biology and ImmunologyUniversity of StuttgartStuttgartGermany

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