FISH With and Without COT1 DNA

  • Vladimir A. TrifonovEmail author
  • Nadezhda N. Vorobieva
  • Willem Rens

Complex FISH probes comprising large spans of genomic DNA always contain a high amount of dispersed repetitive sequences that hamper the visualization of the specific signal of interest. To overcome this problem, different approaches have been elaborated that depend on the type of experiment and the quality of the probe. A classical way to suppress repetitive sequences is to use unlabeled competitor DNA (sheared total genomic DNA or repeated sequence enriched DNA fractions). Here we present two protocols: the first one describes rapid COT DNA isolation and the peculiarities of its use in different FISH experiments, and the second is used for COT-free FISH with complex probes and is based on a special software tool for image enhancement.


Repetitive Sequence Chromosome Paint Yellow Pixel Disperse Repetitive Sequence Image Enhancement Tool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by a grant of the RFBR, DFG research grants to VAT and NVV, and by a Welcome Trust grant to WR.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Vladimir A. Trifonov
    • 1
    Email author
  • Nadezhda N. Vorobieva
    • 1
  • Willem Rens
    • 2
  1. 1.Institute of Cytology and GeneticsRussian Academy of SciencesNovosibirskRussian Federation
  2. 2.Department of Veterinary MedicineUniversity of CambridgeCambridgeUK

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