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Oligonucleotide FISH Probes

  • Sean Bradley
  • Leah Zamechek
  • Joan Aurich-CostaEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

The use of highly specific synthetic oligonucleotide (ODN) probes, which have superior hybridization kinetics and lower manufacturing costs compared to traditional genomic probes, is one approach that can be used to perform chromosome enumeration on interphase nuclei by FISH. ODNs also generally hybridize more rapidly, are more consistent, and are cheaper to manufacture. If highly repetitive sequences are targeted, properly designed synthetic ODN probes may prove to be superior reagents for rapid FISH.

Keywords

Falcon Tube Alpha Satellite Lower Manufacturing Cost Fish Probe Antifade Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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  5. Waye JS, Willard HF (1985) Chromosome-specific alpha satellite DNA: nucleotide sequence analysis of the 2.0 kilobasepair repeat from the human X chromosome. Nucleic Acids Res 13(8):2731–2743CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Sean Bradley
    • 1
  • Leah Zamechek
    • 1
  • Joan Aurich-Costa
    • 1
    Email author
  1. 1.One Cell Systems, Inc.CambridgeUSA

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