FISH on Insect Cells Transfected with Heterologous DNA: A Single-Cell-Directed Approach

  • Thomas Liehr
Part of the Springer Protocols Handbooks book series (SPH)

Insect cells transfected with heterologous DNA can be used to assess the functional properties of genes that have been isolated or subjected to in vitro mutagenesis. An example of such a cell system is embryonic cells derived from Drosophila melanogaster. These cells can be transfected with special plasmids and used for heterologous protein expression. FISH is an elegant technique for monitoring the transfection efficiency at the single-cell level. The background of this method and the approach used are described here in detail.


Bacterial Artificial Chromosome Insect Cell Nick Translation Heterologous Protein Expression Transfection Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Bunch TA, Grinblat Y, Goldstein LS (1988) Characterization and use of the Drosophila metal-lothionein promoter in cultured Drosophila melanogaster cells. Nucleic Acids Res 16:1043–1061CrossRefPubMedGoogle Scholar
  2. Ekici AB, Fuchs C, Nelis E, Hillenbrand R, Schachner M, Van Broeckhoven C, Rautenstrauss B (1998) An adhesion test system based on Schneider cells to determine genotype-phenotype correlations for mutated P0 proteins. Genet Anal 14:117–119PubMedGoogle Scholar
  3. Keith MB, Farrell PJ, Iatrou K, Behie LA (1999) Screening of transformed insect cell lines for recombinant protein production. Biotechnol Prog 15:1046–1052CrossRefPubMedGoogle Scholar
  4. Rautenstrauss B, Fuchs C, Ekici A, Nelis E, Van Broeckhoven C, Liehr T (1998) Assay of trans-fection rate in insect cells on a single cell level. Genet Anal Biomol Eng 14:103–104CrossRefGoogle Scholar
  5. Rubin GM, Spradling AC (1983) Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res 11:6341–6351CrossRefPubMedGoogle Scholar
  6. Schneider I (1972) New cell line from embryonic cells of Drosophila melanogaster. J Embr Exp Morph 27:353–356Google Scholar
  7. Snow PM, Bieber AJ, Goodman CS (1989) Fasciclin III: a novel homophilic adhesion molecule in Drosophila. Cell 59:313–323CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Thomas Liehr
    • 1
  1. 1.Institut für Humangenetik und Anthropologie, Kollegiengasse 10JenaGermany

Personalised recommendations