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FISH on Insect Cells Transfected with Heterologous DNA: A Single-Cell-Directed Approach

  • Thomas Liehr
Protocol
  • 1.6k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Insect cells transfected with heterologous DNA can be used to assess the functional properties of genes that have been isolated or subjected to in vitro mutagenesis. An example of such a cell system is embryonic cells derived from Drosophila melanogaster. These cells can be transfected with special plasmids and used for heterologous protein expression. FISH is an elegant technique for monitoring the transfection efficiency at the single-cell level. The background of this method and the approach used are described here in detail.

Keywords

Bacterial Artificial Chromosome Insect Cell Nick Translation Heterologous Protein Expression Transfection Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Thomas Liehr
    • 1
  1. 1.Institut für Humangenetik und Anthropologie, Kollegiengasse 10JenaGermany

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