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Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid

  • Nicole Müller-Sienerth
  • Cécile Crosnier
  • Gavin J. Wright
  • Nicole Staudt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1131)

Abstract

Antibodies are an integral part of biological and medical research. In addition, immunoglobulins are used in many diagnostic tests and are becoming increasingly important in the therapy of diseases. To express antibodies recombinantly, the immunoglobulin heavy and light chains are usually cloned into two different expression plasmids. Here, we describe a method for recombinant antibody expression from a single plasmid.

Key words

Recombinant antibodies Monoclonal antibodies Hybridomas Single expression plasmid Fusion PCR 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Nicole Müller-Sienerth
    • 1
  • Cécile Crosnier
    • 1
  • Gavin J. Wright
    • 1
  • Nicole Staudt
    • 1
  1. 1.Cell Surface Signalling LaboratoryWellcome Trust Sanger InstituteHinxtonUK

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