The Murine Femoral Bone Graft Model and a Semiautomated Histomorphometric Analysis Tool

  • Robinder S. Dhillon
  • Longze Zhang
  • Edward M. Schwarz
  • Brendan F. Boyce
  • Chao Xie
Part of the Methods in Molecular Biology book series (MIMB, volume 1130)


Preclinical studies of bone repair remain a high priority because of unresolved clinical problems associated with treating critical segmental defects and complications of fracture healing. Over the last decade, the murine femoral allograft model has gained popularity due to its standardized surgery and potential for examining a vast array of radiographic, biomechanical, and histological outcome measures. Here, we describe these methods and a novel semiautomated histomorphometric approach to quantify the amount of bone, cartilage, and undifferentiated mesenchymal tissue in demineralized paraffin sections of allografted murine femurs using the Visiopharm Image Analysis Software System.


Visiopharm Histomorphometry Bone graft Allograft Autograft Skeletal repair 



The authors would like to thank Ryan Tierney, Sarah Mack, Kathleen Maltby, and Ashish Thomas for their assistance with the histology and whole-slide scanning. This work was supported by research grants from the National Institutes of Health (1S10RR027340, DE19902, AR54041, and P30 AR061307).


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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Robinder S. Dhillon
    • 1
  • Longze Zhang
    • 1
  • Edward M. Schwarz
    • 1
  • Brendan F. Boyce
    • 1
  • Chao Xie
    • 1
  1. 1.University of Rochester Medical CenterRochesterUSA

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