In Vivo Protein–Protein Interaction Studies with BiFC: Conditions, Cautions, and Caveats
Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein–protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.
Key wordsSplit-YFP BiFC Agroinfiltration
This work was supported by BBSRC and the Scottish Government’s Rural and Environment Science and Analytical Services (RESAS) Division.
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