In Vivo Protein–Protein Interaction Studies with BiFC: Conditions, Cautions, and Caveats

  • Petra Boevink
  • Hazel McLellan
  • Tatyana Bukharova
  • Stefan Engelhardt
  • Paul Birch
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1127)

Abstract

Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein–protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.

Key words

Split-YFP BiFC Agroinfiltration 

Notes

Acknowledgment

This work was supported by BBSRC and the Scottish Government’s Rural and Environment Science and Analytical Services (RESAS) Division.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Petra Boevink
    • 1
    • 2
  • Hazel McLellan
    • 2
    • 3
  • Tatyana Bukharova
    • 2
    • 3
  • Stefan Engelhardt
    • 2
    • 3
  • Paul Birch
    • 1
    • 2
    • 3
  1. 1.Cell and Molecular Sciences, The James Hutton InstituteInvergowrie, DundeeUK
  2. 2.Dundee Effector Consortium, The James Hutton InstituteInvergowrie, DundeeUK
  3. 3.Division of Plant SciencesCollege of Life Sciences, University of Dundee (at JHI)Invergowrie, DundeeUK

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