Genomic DNA Library Preparation for Resistance Gene Enrichment and Sequencing (RenSeq) in Plants

  • Florian Jupe
  • Xinwei Chen
  • Walter Verweij
  • Kamel Witek
  • Jonathan D. G. Jones
  • Ingo Hein
Part of the Methods in Molecular Biology book series (MIMB, volume 1127)


Enrichment of genomic DNA for genes of interest prior to high-throughput sequencing offers an efficient and cost-effective approach to reduce genome complexity. Target enrichment typically yields higher read-depth for selected genes and is therefore suitable for determination of sequence polymorphisms and enables multiplexing of samples. Target enrichment also provides a means to annotate specific gene families within the sequenced organisms without the requirements for gene models. Here we describe enrichment procedures for NB-LRR-type plant resistance genes that can, for example, be used to establish the NB-LRR gene complements of individual plants and to map resistances more rapidly using a bulked segregant analysis.

Key words

Agilent SureSelect Target enrichment High-throughput sequencing NB-LRR genes 


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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.The Sainsbury LaboratoryNorwich Research ParkNorwichUK
  2. 2.CMS and Dundee Effector ConsortiumThe James Hutton InstituteInvergowrie DundeeUK
  3. 3.The Genome Analysis CentreNorwichUK

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