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Secondary Ion Mass Spectrometry Imaging of Biological Cells and Tissues

  • Nicholas P. Lockyer
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1117)

Abstract

Secondary ion mass spectrometry (SIMS) is capable of providing detailed atomic and molecular characterization of the surface chemistry of (bio)molecular samples. It is one of a range of mass spectrometry imaging techniques that combine the high sensitivity and specificity of mass spectrometry with the capability to view the distribution of analytes within solid samples. The technique is particularly suited to the detection and imaging of small molecules such as lipids and other metabolites. A limit of detection in the ppm range and spatial resolution <1 μm can be obtained. Recent progress in instrumental developments, including new cluster ion beams, the implementation of tandem mass spectrometry (MS/MS), and the application of multivariate data analysis protocols promise further advances. This chapter presents a brief overview of the technique and methodology of SIMS using exemplar studies of biological cells and tissue.

Key words

ToF-SIMS Imaging Mass spectrometry Cellular analysis Tissue analysis Molecular depth profiling 3D SIMS 

Notes

Acknowledgements

The author would like to thank Prof. J.C. Vickerman and members of our research group for their contribution. The financial support of the Engineering and Physical Sciences Research Council (EPSRC) and Biotechnology and Biological Sciences Research Council (BBSRC) is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Nicholas P. Lockyer
    • 1
  1. 1.Manchester Institute of BiotechnologyUniversity of ManchesterManchesterUK

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