Seamless Ligation Cloning Extract (SLiCE) Cloning Method

  • Yongwei Zhang
  • Uwe Werling
  • Winfried Edelmann
Part of the Methods in Molecular Biology book series (MIMB, volume 1116)


SLiCE (Seamless Ligation Cloning Extract) is a novel cloning method that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of traditional cloning methods, facilitates seamless cloning by recombining short end homologies (15–52 bp) with or without flanking heterologous sequences and provides an effective strategy for directional subcloning of DNA fragments from bacterial artificial chromosomes or other sources. SLiCE is highly cost-effective and demonstrates the versatility as a number of standard laboratory bacterial strains can serve as sources for SLiCE extract. We established a DH10B-derived E. coli strain expressing an optimized λ prophage Red recombination system, termed PPY, which facilitates SLiCE with very high efficiencies.

Key words

SLiCE cloning In vitro recombination Seamless cloning Bacterial cell extract DNA cloning Recombinant DNA 



This work was supported by National Institute of Health [1R01CA76329 to W.E., 1R01CA93484 to W.E].


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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Yongwei Zhang
    • 1
  • Uwe Werling
    • 1
  • Winfried Edelmann
    • 1
  1. 1.Department of Cell BiologyAlbert Einstein College of MedicineBronxUSA

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