Chemical Modification of the Inner and Outer Surfaces of Tobacco Mosaic Virus (TMV)

  • Michael A. Bruckman
  • Nicole F. Steinmetz
Part of the Methods in Molecular Biology book series (MIMB, volume 1108)


Viral nanoparticles derived from tobacco mosaic virus (TMV) find applications in various fields. We report the purification and chemical modification of TMV which is a hollow rod-shaped plant viral nanoparticle with modifiable interior and exterior surfaces. We describe methods to isolate TMV from its tobacco plant host for spatially controlled interior and exterior chemical modification and to characterize the resulting TMV hybrid materials.

Key words

Bioconjugation chemistry CuAAC (click) chemistry Tobacco mosaic virus (TMV) Viral nanoparticles (VNPs) 



This work was supported by Mt. Sinai Foundation (to NFS), and NIH/NHLBI T32 5T32HL105338-03 training grant (to MAB).


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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Michael A. Bruckman
    • 1
  • Nicole F. Steinmetz
    • 2
    • 3
    • 4
  1. 1.Departments of Biomedical EngineeringCase Western Reserve UniversityClevelandUSA
  2. 2.Department of Biomedical EngineeringCase Western Reserve UniversityClevelandUSA
  3. 3.Department of RadiologyCase Western Reserve UniversityClevelandUSA
  4. 4.Department of Materials Science and EngineeringCase Western Reserve UniversityClevelandUSA

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