Detection of Cell Membrane-Bound CD46 Using Flow Cytometry

  • Martin Kolev
  • Claudia Kemper
Part of the Methods in Molecular Biology book series (MIMB, volume 1100)


CD46 is an important regulator of the complement system by preventing unwanted deposition of the complement activation products and opsonins C3b/C4b onto self-tissue. Recently, intracellular signals mediated by CD46 activation on several distinct human cell types have demonstrated that CD46 also plays decisive roles in immuneregulation. The growing recognition of CD46 as key regulator in several vital biological processes, led to increased demand in sensitive methods for monitoring CD46 expression and changes thereof on cells and in tissues. Here we describe a method, which allows for studying CD46 expression on the surface of cells using specific antibodies in combination with fluorescence-activated cell sorting (FACS) analysis.

Key words

CD46 MCP Complement Flow cytometry Antibodies 



This work is supported by an MRC Research Grant (Grant no. G1002165 awarded to CK), the Medical Research Council Centre for Transplantation, Guy’s Hospital, King’s College and the Department of Health, National Institute for Health Research comprehensive Biomedical Research Centre award to Guy’s & St. Thomas’ NHS Foundation Trust in partnership with King’s College London and King’s College Hospital NHS Foundation Trust.


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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Martin Kolev
    • 1
  • Claudia Kemper
    • 1
  1. 1.Division of Transplantation Immunology and Mucosal Biology, MRC Centre for TransplantationKing’s College London, Guy’s HospitalLondonUK

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