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DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)

  • Chiaki Mutou
  • Katsunori Tanaka
  • Ryuji Ishikawa
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1099)

Abstract

Deoxyribonucleic acid (DNA) extracted from endosperm can be effectively used for rapid genotyping using seed tissue, to evaluate seed quality from packaged grains and to determine the purity of milled grains. Methods outlined here are optimal procedures to isolate DNA from endosperm tissue of modern rice grains and of aged rice remains preserved between 50 and 100 years. The extracted DNA can be used to amplify regions of chloroplast genomic DNA (ctDNA), mitochondrial genomic DNA (mtDNA), and nuclear genomic DNA using standard PCR protocols. In addition, we describe an optimal procedure to process archaeological grain specimens, aged for a couple of thousand years, to isolate DNA from these ancient samples, referred to here as ancient DNA (aDNA). The aDNA can be successfully amplified by PCR using appropriate primer pairs designed specifically for aDNA amplification.

Keywords

Endosperm Urea Organellar DNA nDNA Ancient DNA 

References

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    Pääbo S, Poinar H, Serre D et al (2004) Genetic analyses from ancient DNA. Annu Rev Genet 38:645–679PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Chiaki Mutou
    • 1
  • Katsunori Tanaka
    • 2
  • Ryuji Ishikawa
    • 3
  1. 1.National Institute of Aerobiological SciencesTsukubaJapan
  2. 2.Faculty of HumanitiesHirosaki UniversityHirosakiJapan
  3. 3.Faculty of Agriculture and Life ScienceHirosaki UniversityHirosakiJapan

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