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DNA Extraction from Vegetative Tissue for Next-Generation Sequencing

  • Agnelo Furtado
Part of the Methods in Molecular Biology book series (MIMB, volume 1099)

Abstract

The quality of extracted DNA is crucial for several applications in molecular biology. If the DNA is to be used for next-generation sequencing (NGS), then microgram quantities of good-quality DNA is required. In addition, the DNA must substantially be of high molecular weight so that it can be used for library preparation and NGS sequencing. Contaminating phenol or starch in the isolated DNA can be easily removed by filtration through kit-based cartridges. In this chapter we describe a simple two-reagent DNA extraction protocol which yields a high quality and quantity of DNA which can be used for different applications including NGS.

Keywords

DNA Plant Starch contamination High molecular weight Next-generation sequencing 

Reference

  1. 1.
    Carroll BJ, Klimyuk VI, Thomas CM et al (1995) Germinal transpositions of the maize element dissociation from T-DNA loci in tomato. Genetics 139:407–420PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Agnelo Furtado
    • 1
  1. 1.Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandSt. LuciaAustralia

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