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Isolation of Zebrafish Gonads for RNA Isolation

  • Hsin-Yi Huang
  • René F. Ketting
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1093)

Abstract

Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.

Key words

Zebrafish piRNA Germline development Gonads Ovary Testis RNA FACS Vasa 

Notes

Acknowledgments

We thank Stefan Redl for help in making figures. This work was supported by VIDI (864.05.010), ECHO (700.57.006), and VICI (724.011.001) grants from the Netherlands Organization for Scientific Research.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Hsin-Yi Huang
    • 1
  • René F. Ketting
    • 2
  1. 1.Ontario Cancer InstitutePrincess Margaret HospitalTorontoCanada
  2. 2.Institute of Molecular Biology (IMB), Faculty of BiologyUniversity of MainzMainzGermany

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