PIWI-Interacting RNAs pp 1-11

Part of the Methods in Molecular Biology book series (MIMB, volume 1093) | Cite as

Chromatin Immunoprecipitation Assay of Piwi in Drosophila

  • Hang Yin
  • Haifan Lin
Protocol

Abstract

The generation of high-resolution maps of the epigenome is crucial to research in epigenetics, developmental biology, and stem cell biology. In recent years, small RNA pathways have been implicated in epigenetic regulation. All small RNA pathways involve Argonaute proteins as their key biogenesis and effector components. In this chapter, we describe a chromatin immunoprecipitation method for whole-genome mapping of Drosophila Piwi, the defining member of the Argonaute protein family. This method should have general utility for mapping other chromatin-associated factors.

Key words

Piwi Chromatin immunoprecipitation assay Epigenetics 

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Hang Yin
    • 1
  • Haifan Lin
    • 2
  1. 1.Ottawa Hospital Research InstituteOttawaCanada
  2. 2.Department of Cell BiologyYale Stem Cell Center, Yale School of MedicineNew HavenUSA

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