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In Vivo NMR for 13C metabolic Flux Analysis

  • Albrecht Roscher
  • Stéphanie Troufflard
  • Abdelghani Idrissi Taghki
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1090)

Abstract

The use of in vivo NMR within the framework of Metabolic Flux Analysis in plants is presented. In vivo NMR allows to visualize the active metabolic network, to determine metabolic and isotopic steady state and to measure metabolic fluxes which are not necessarily accessible by isotopic steady state (stationary) Metabolic Flux Analysis. The kinetic data can be used as input for dynamic (nonstationary) Metabolic Flux Analysis. Both 1D and 2D NMR methods are employed.

Key words

In vivo NMR Plant metabolic NMR 13C NMR Kinetic NMR Metabolic flux analysis 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Albrecht Roscher
    • 1
  • Stéphanie Troufflard
    • 2
  • Abdelghani Idrissi Taghki
    • 3
  1. 1.Génie Enzymatique et Cellulaire, FRE CNRS 3580Université de PicardieAmiens CedexFrance
  2. 2.Département de PhysiqueEcole Normale SupérieureParis CedexFrance
  3. 3.Génie Enzymatique et Cellulaire, UMR CNRS 6022Université de Technologie de CompiègneCompiègne CedexFrance

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