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Construction of a Filamentous Phage Display Peptide Library

  • Annette Fagerlund
  • Astrid Hilde Myrset
  • Mari Ann Kulseth
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1088)

Abstract

The concept of phage display is based on insertion of random oligonucleotides at an appropriate location within a structural gene of a bacteriophage. The resulting phage will constitute a library of random peptides displayed on the surface of the bacteriophages, with the encoding genotype packaged within each phage particle. Using a phagemid/helper phage system, the random peptides are interspersed between wild-type coat proteins. Libraries of phage-expressed peptides may be used to search for novel peptide ligands to target proteins. The success of finding a peptide with a desired property in a given library is highly dependent on the diversity and quality of the library. The protocols in this chapter describe the construction of a high-diversity library of phagemid vector encoding fusions of the phage coat protein pVIII with random peptides, from which a phage library displaying random peptides can be prepared.

Key words

Phage display Phagemid pVIII Peptide library Filamentous phage 

Notes

Acknowledgement

This work was performed at and supported by Medical Diagnostics Research, GE Healthcare.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Annette Fagerlund
    • 1
  • Astrid Hilde Myrset
    • 2
  • Mari Ann Kulseth
    • 3
  1. 1.Department of Pharmaceutical BiosciencesUniversity of OsloOsloNorway
  2. 2.Omegatri AS, Oslo Innovation CentreOsloNorway
  3. 3.Department of Medical Genetics, RikshospitaletOslo University HospitalOsloNorway

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