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Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells

  • Christine Rouzioux
  • Adeline Mélard
  • Véronique Avéttand-Fénoël
Part of the Methods in Molecular Biology book series (MIMB, volume 1087)

Abstract

HIV reservoir measurement in patients is one of the challenges at the time of testing new treatment approaches aiming at eradicating HIV infection. HIV reservoirs are complex and disseminated in a large number of organs and lymphoid tissues. We chose to quantify total cell-associated HIV-DNA in PBMC as a marker of HIV reservoirs and described the method we developed. The marker was used in large cohort studies at different stages of HIV disease and in therapeutical trials. Our results show how informative is this marker, as well as that plasma HIV-RNA and CD4 T cell count are representative of each patient when measured in blood. Such a series of results might help to adapt simplification or structured interruption strategies, design new clinical trials targeting HIV reservoirs, and select populations that could benefit of such new treatments.

Key words

HIV-DNA Quantification Real-time PCR Reservoirs 

Notes

Acknowledgments

Our work is supported by grants from the ANRS (Agence Nationale de Recherches sur le Sida et les Hépatites virales) and the Paris Descartes University. A. M is supported by the ANRS.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Christine Rouzioux
    • 1
    • 2
  • Adeline Mélard
    • 1
    • 3
  • Véronique Avéttand-Fénoël
    • 1
    • 4
  1. 1.Laboratoire de VirologieHôpital NeckerParisFrance
  2. 2.EA 3620, Paris Descartes UniversityParisFrance
  3. 3.EA 3620, Université Paris DescartesSorbonne Paris-CitéFrance
  4. 4.EA 3620 Université Paris DescartesSorbonne Paris-CitéFrance

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