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RNA Folding pp 309-319 | Cite as

RNA Refolding Studied by Light-Coupled NMR Spectroscopy

  • Harald Schwalbe
  • Boris Fürtig
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1086)

Abstract

Conformational transitions (refolding) between long-lived conformational states constitute the time-limiting step during the folding process of large RNAs. As the dynamics of these reactions dominate the regulatory and other functional behavior of RNA molecules, it is of importance to characterize them with high spatial and temporal resolution. Here, we describe a method for the investigation of RNA refolding reactions based on the photolytic generation of preselected conformations in a non-equilibrium state, followed by the observation of the folding trajectory with real-time NMR spectroscopy.

Key words

Real-time NMR NMR laser coupling RNA refolding Photo-cleavable protecting groups Long-lived conformational states 

Notes

Acknowledgements

The work is supported by the state of Hesse (BMRZ), the DFG collaborative research center: Molecular principles of RNA-based regulation. H.S. is a member of the DFG center of excellence: macromolecular complexes.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Harald Schwalbe
    • 1
  • Boris Fürtig
    • 1
  1. 1.Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic ResonanceJohann Wolfgang Goethe-UniversityFrankfurt am MainGermany

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