RNA Refolding Studied by Light-Coupled NMR Spectroscopy
Conformational transitions (refolding) between long-lived conformational states constitute the time-limiting step during the folding process of large RNAs. As the dynamics of these reactions dominate the regulatory and other functional behavior of RNA molecules, it is of importance to characterize them with high spatial and temporal resolution. Here, we describe a method for the investigation of RNA refolding reactions based on the photolytic generation of preselected conformations in a non-equilibrium state, followed by the observation of the folding trajectory with real-time NMR spectroscopy.
Key wordsReal-time NMR NMR laser coupling RNA refolding Photo-cleavable protecting groups Long-lived conformational states
The work is supported by the state of Hesse (BMRZ), the DFG collaborative research center: Molecular principles of RNA-based regulation. H.S. is a member of the DFG center of excellence: macromolecular complexes.
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