The Cre/Lox System to Assess the Development of the Mouse Brain

  • Claudius F. Kratochwil
  • Filippo M. Rijli
Part of the Methods in Molecular Biology book series (MIMB, volume 1082)


Cre-mediated recombination has become a powerful tool to confine gene deletions (conditional knockouts) or overexpression of genes (conditional knockin/overexpression). By spatiotemporal restriction of genetic manipulations, major problems of classical knockouts such as embryonic lethality can be circumvented. Furthermore Cre-mediated recombination has broad applicability in the analysis of the cellular behavior of subpopulations and cell types as well as for genetic fate mapping. This chapter will give an overview about applications for the Cre/LoxP system and their execution.

Key words

Cre recombinase Transgenesis Conditional knockout Conditional knockin CreERT2 Flpe recombinase MADM Split-Cre Brainbow 



Work in F.M.R. laboratory in Friedrich Miescher Institute (Basel, Switzerland) is supported by the Swiss National Science Foundation (Sinergia CRSI33_127440), ARSEP, and the Novartis Research Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Claudius F. Kratochwil
    • 1
  • Filippo M. Rijli
    • 1
  1. 1.Friedrich Miescher Institute for Biomedical ResearchBaselSwitzerland

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