Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
Antisense oligodeoxynucleotide AODN Pollen tube Gene knockdown Tip growth
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Pollen tube research in the Žárský lab is supported by the Czech Grant Agency grant GACR 13-19073S to M. P.
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