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Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1080)

Abstract

Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.

Key words

Antisense oligodeoxynucleotide AODN Pollen tube Gene knockdown Tip growth 

Notes

Acknowledgment

Pollen tube research in the Žárský lab is supported by the Czech Grant Agency grant GACR 13-19073S to M. P.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Department of Experimental Plant Biology, Faculty of ScienceCharles University and Institute of Experimental Botany, Academy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPragueCzech Republic

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