Plant Cell Lines in Cell Morphogenesis Research
Plant organs and tissues consist of many various cell types, often in different phases of their development. Such complex structures do not allow direct studies on behavior of individual cells. In contrast, populations of in vitro-cultured plant cells represent valuable tool for studying processes on a single-cell level, including cell morphogenesis. Here we describe characteristics of well-established model tobacco and Arabidopsis cell lines and provide detailed protocol on their cultivation, characterization, and genetic transformation.
Key wordsBY-2 VBI-0 Suspension-cultured cells Cell phenotyping Cell viability Cell density Culture friability Micromorphology Subculture interval (SBI)
The authors acknowledge support for their work by the Grant Agency of the Czech Republic (projects P305/11/0797 and P305/11/2476), Ministry of Education, Youth and Sport of the Czech Republic (project MSM00216208858), and Charles University in Prague (project SVV 265203/2012).
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