Sirtuins pp 203-215

Part of the Methods in Molecular Biology book series (MIMB, volume 1077) | Cite as

Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography

  • Jun Yoshino
  • Shin-ichiro Imai
Protocol

Abstract

Nicotinamide adenine dinucleotide (NAD+) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD+ levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD+ measurement, which enables us to analyze various pathophysiological changes in NAD+ levels in vitro and in vivo.

Key words

Nicotinamide adenine dinucleotide (NAD+HPLC NAMPT 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Jun Yoshino
    • 1
  • Shin-ichiro Imai
    • 2
  1. 1.Center for Human Nutrition, Department of MedicineWashington University School of MedicineSt. LouisUSA
  2. 2.Department of Developmental BiologyWashington University School of MedicineSt. LouisUSA

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