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Genetically Encoded FRET Indicators for Live-Cell Imaging of Histone Acetylation

  • Kazuki Sasaki
  • Minoru Yoshida
Part of the Methods in Molecular Biology book series (MIMB, volume 1071)

Abstract

Histone acetylation is dynamically and reversibly controlled by histone acetyltransferases and deacetylases during cellular events such as cell division and differentiation. However, the dynamics of histone modifications in living cells are poorly understood because of the lack of experimental tools to monitor them in a real-time fashion. Herein, we introduce Förster/fluorescence resonance energy transfer (FRET)-based indicators to visualize acetylation of histone H4, and describe a protocol for live-cell imaging with high spatiotemporal resolution.

Key words

Histone acetylation Bromodomain Förster/fluorescence resonance energy transfer (FRET) Live-cell imaging Fluorescent protein 

Notes

Acknowledgments

This work was supported by JST, PRESTO.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Kazuki Sasaki
    • 1
  • Minoru Yoshida
    • 2
    • 3
  1. 1.Chemical Genetics Laboratory/Chemical Genomics Research Group, RIKEN Advanced Science InstituteWakoJapan
  2. 2.Chemical Genetics Laboratory/Chemical Genomics Research Group, RIKEN Advanced Science InstituteGraduate School of Science and Engineering, Saitama UniversitySaitamaJapan
  3. 3.CREST Research ProjectJapan Science and Technology CorporationKawaguchiJapan

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