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Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans

  • Scott J. Neal
  • Kyuhyung Kim
  • Piali Sengupta
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1068)

Abstract

Environmental conditions experienced during early larval stages dictate the developmental trajectory of the nematode C. elegans. Favorable conditions such as low population density, abundant food, and lower temperatures allow reproductive growth, while stressful conditions promote entry of second-stage (L2) larvae into the alternate dauer developmental stage. Population density is signaled by the concentration and composition of a complex mixture of small molecules that is produced by all stages of animals, and is collectively referred to as dauer pheromone; pheromone concentration is a major trigger for dauer formation. Here, we describe a quantitative dauer formation assay that provides a measure of the potency of single or mixtures of pheromone components in regulating this critical developmental decision.

Key words

Dauer larva Ascaroside C. elegans Pheromone 

Notes

Acknowledgments

Funding for this work was provided by the National Science Foundation (IOS 0842452 and IOS 1256488 to P.S.), the Human Frontiers Science Program (RGY0042/2010 to P.S.), and the DGIST Convergence Science Center Program of the Ministry of Education, Science and Technology (11-BD-04 to K.K.). S.J.N. was supported by a postgraduate scholarship (PGS-D3) from the Natural Sciences and Engineering Research Council of Canada and by the Brandeis National Committee.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Scott J. Neal
    • 1
  • Kyuhyung Kim
    • 2
  • Piali Sengupta
    • 1
    • 3
  1. 1.Department of BiologyBrandeis UniversityWalthamUSA
  2. 2.Department of Brain ScienceDaegu Gyeongbuk Institute of Science and Technology (DGIST)DaeguSouth Korea
  3. 3.National Center for Behavioral GenomicsBrandeis UniversityWalthamUSA

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