Using a Reverse Genetics Approach to Investigate Small-Molecule Activity

  • Siamsa M. Doyle
  • Stéphanie Robert
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1056)

Abstract

Chemical genomics is a highly effective approach for understanding complex and dynamic biological processes in plants. A chemical activity can be investigated by a reverse genetics strategy, for which a huge abundance and diversity of Arabidopsis thaliana mutants are readily available for exploitation. Here we present an approach to characterize a chemical of interest, as well as examples of studies demonstrating an effective combination of chemical genomics with reverse genetics strategies, drawn from recent literature on phytohormone signalling and auxin transport.

Key words

Chemical genomics Arabidopsis mutants Reverse genetics Phytohormone signalling Auxin transport 
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Notes

Acknowledgements

We thank the Vetenskapsrådet and VINNOVA for supporting this work.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Siamsa M. Doyle
    • 1
  • Stéphanie Robert
    • 1
  1. 1.Department of Forest Genetics and Plant Physiology, Umeå Plant Science CentreSwedish University of Agricultural Sciences (SLU)UmeåSweden

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