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An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus

  • Kevin M. Downard
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1061)

Abstract

The structure and antigenicity of protein antigens of the influenza virus are screened in a single step employing an immunoproteomics approach. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) coupled to gel electrophoresis is used both to identify viral antigens and screen their antigenicity. Earlier evidence that antigen–antibody complexes can survive on MALDI targets has allowed both the primary structure and antigenicity of viral strains to be rapidly screened with the specific localization of protein epitopes. The approach is anticipated to have a greater role in the future surveillance of the virus and should also aid in the development of immunogenic peptide constructs as alternatives to whole virus for vaccination.

Key words

Influenza Flu Virus Surveillance Antigenicity Mass spectrometry Proteomics Immunoproteomics 

Notes

Acknowledgments

The author acknowledges the contributions of Bethny Morrissey, Dr. Alexander Schwahn, Dr. Margaret Streamer, and Joshua Ho as indicated by the cited works. The author thanks Robert Shaw and Dr. Ian Barr of the WHO Collaborating Centre for Reference and Research on Influenza and Dr. Elizabeth Pietrzykowski of CSL Limited for the supply of some virus strains and monoclonal antibodies, respectively. Funding for this work was provided by the Australian Research Council (DP0449800 and DP0770619 to the author) and the University of Sydney.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Kevin M. Downard
    • 1
  1. 1.School of Molecular BioscienceUniversity of SydneySydneyAustralia

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