Antigen Identification Using SEREX

  • Ugur Sahin
  • Özlem Türeci
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1061)

Abstract

Serological analysis of recombinant cDNA expression libraries (SEREX) allows systematic identification of antigens recognized by the spontaneous autoantibody repertoire of patients with cancer and autoimmune disease. SEREX is based on screening of lambda phage expression libraries constructed from diseased tissue with the autologous patient’s serum. This chapter provides the key protocols for SEREX immunoscreening and for downstream validation of autoantigens.

Key words

SEREX Autoantibodies Serology Autoantigens Expression cloning Immunoproteomics Biomarker 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Ugur Sahin
    • 1
  • Özlem Türeci
    • 2
  1. 1.Translational Oncology (TRON)MainzGermany
  2. 2.Translational Oncology at the University HospitalMainzGermany

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