Membrane Protein Structure Determination: Back to the Membrane

  • Yong Yao
  • Yi Ding
  • Ye Tian
  • Stanley J. Opella
  • Francesca M. Marassi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1063)

Abstract

NMR spectroscopy enables the structures of membrane proteins to be determined in the native-like environment of the phospholipid bilayer membrane. This chapter outlines the methods for membrane protein structural studies using solid-state NMR spectroscopy with samples of membrane proteins incorporated in proteoliposomes or planar lipid bilayers. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from OmpX and Ail, two bacterial outer membrane proteins that function in bacterial virulence.

Key words

Membrane protein NMR Lipid Bilayer Membrane Protein Expression Structure Barrel 

Notes

Acknowledgments

This research was supported by grants from the National Institutes of Health (R21 GM075917; R21 GM094727; R01 GM100265). The NMR studies utilized the NMR Facility at Sanford-Burnham Medical Research Institute, and the Resource for Molecular Imaging of Proteins at UCSD, each supported by grants from the National Institutes of Health (P30 CA030199; P41 EB002031).

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Yong Yao
    • 1
  • Yi Ding
    • 1
  • Ye Tian
    • 1
    • 2
  • Stanley J. Opella
    • 2
  • Francesca M. Marassi
    • 1
  1. 1.Sanford Burnham Medical Research InstituteLa JollaUSA
  2. 2.Department of Chemistry and BiochemistryUniversity of California, San DiegoLa JollaUSA

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