Protocol

Membrane Proteins

Volume 1063 of the series Methods in Molecular Biology pp 145-158

Date:

Membrane Protein Structure Determination: Back to the Membrane

  • Yong YaoAffiliated withSanford Burnham Medical Research Institute
  • , Yi DingAffiliated withSanford Burnham Medical Research Institute
  • , Ye TianAffiliated withSanford Burnham Medical Research InstituteDepartment of Chemistry and Biochemistry, University of California, San Diego
  • , Stanley J. OpellaAffiliated withDepartment of Chemistry and Biochemistry, University of California, San Diego
  • , Francesca M. MarassiAffiliated withSanford Burnham Medical Research Institute

* Final gross prices may vary according to local VAT.

Get Access

Abstract

NMR spectroscopy enables the structures of membrane proteins to be determined in the native-like environment of the phospholipid bilayer membrane. This chapter outlines the methods for membrane protein structural studies using solid-state NMR spectroscopy with samples of membrane proteins incorporated in proteoliposomes or planar lipid bilayers. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from OmpX and Ail, two bacterial outer membrane proteins that function in bacterial virulence.

Key words

Membrane protein NMR Lipid Bilayer Membrane Protein Expression Structure Barrel