Growing Arabidopsis In Vitro: Cell Suspensions, In Vitro Culture, and Regeneration

  • Bronwyn J. Barkla
  • Rosario Vera-Estrella
  • Omar Pantoja
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1062)

Abstract

An understanding of basic methods in Arabidopsis tissue culture is beneficial for any laboratory working on this model plant. Tissue culture refers to the aseptic growth of cells, organs, or plants in a controlled environment, in which physical, nutrient, and hormonal conditions can all be easily manipulated and monitored. The methodology facilitates the production of a large number of plants that are genetically identical over a relatively short growth period. Techniques, including callus production, cell suspension cultures, and plant regeneration, are all indispensable tools for the study of cellular biochemical and molecular processes. Plant regeneration is a key technology for successful stable plant transformation, while cell suspension cultures can be exploited for metabolite profiling and mining. In this chapter we report methods for the successful and highly efficient in vitro regeneration of plants and production of stable cell suspension lines from leaf explants of both Arabidopsis thaliana and Arabidopsis halleri.

Key words

Callus Cell suspensions Plant regeneration Tissue culture Arabidopsis Organ regeneration 

Notes

Acknowledgments

Work in the authors’ lab is funded by DGAPA IN203913 to B.J.B., DGAPA 203711 to R.V.-E., and DGAPA 203112 and CONACyT IN79191 to O.P.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Bronwyn J. Barkla
    • 1
  • Rosario Vera-Estrella
    • 1
  • Omar Pantoja
    • 1
  1. 1.Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavacaMexico

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