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Live Imaging of Arabidopsis Development

  • Daniel von Wangenheim
  • Gabor Daum
  • Jan U. Lohmann
  • Ernst K. Stelzer
  • Alexis Maizel
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1062)

Abstract

Live cell imaging is an essential methodology for studying the structure, dynamics, and functions of cells in a living plant under normal or stressed growth conditions. Arabidopsis thaliana is perfectly amenable to various live microscopy techniques. In this chapter, we provide guidelines to design live-imaging experiments. We discuss specifically the respective advantage of each microscopy technique, the choice of reporter, and the preparation of the sample. Detailed protocols for imaging of shoot and roots are provided.

Key words

Confocal microscopy Light sheet microscopy 4D imaging Fluorescence 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Daniel von Wangenheim
    • 1
  • Gabor Daum
    • 2
    • 3
  • Jan U. Lohmann
    • 2
    • 3
  • Ernst K. Stelzer
    • 1
  • Alexis Maizel
    • 3
  1. 1.Physical Biology, Frankfurt Institute for Molecular Life Sciences (FMLS)Goethe Universität Frankfurt am MainFrankfurt am MainGermany
  2. 2.Department of Stem Cell BiologyUniversity of HeidelbergHeidelbergGermany
  3. 3.Centre for Organismal StudiesUniversity of HeidelbergHeidelbergGermany

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