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Applications of Fluorescent Marker Proteins in Plant Cell Biology

  • Michael R. Blatt
  • Christopher Grefen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1062)

Abstract

Over the past decade, confocal microscopy and the ever-expanding toolchest of fluorescent protein (xFP) markers and technologies have become routine methods for the biological laboratory. A common use of xFP fluorophores is in localizing proteins and the subcellular structures with which they associate, including analyzing their distribution and dynamics and the interactions of proteins in vivo. Additionally, a number of so-called optical highlighters have proven especially useful in analyzing the kinetics of these processes in pulse-chase studies of protein relocation(s) following an experimental challenge. Here we focus on exemplary methods in transformation and live-cell imaging in plant cells, with the expectation that researchers will find these and the accompanying resources useful as a starting point in developing their own expertise.

Key words

Confocal fluorescence microscopy Fluorescent marker proteins Optical highlighter proteins Transient Arabidopsis transformation Quantitative ratiometric fluorescence 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Michael R. Blatt
    • 1
  • Christopher Grefen
    • 2
  1. 1.Laboratory of Plant Physiology and BiophysicsUniversity of GlasgowGlasgowUK
  2. 2.Centre for Molecular Biology of Plants (ZMBP), Developmental GeneticsUniversity of TübingenTübingenGermany

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