Application of DNA Microarray Technology to Gerontological Studies

  • Kiyoshi Masuda
  • Yuki Kuwano
  • Kensei Nishida
  • Kazuhito Rokutan
Part of the Methods in Molecular Biology book series (MIMB, volume 1048)


Gene expression patterns change dramatically in aging and age-related events. The DNA microarray is now recognized as a useful device in molecular biology and widely used to identify the molecular mechanisms of aging and the biological effects of drugs for therapeutic purpose in age-related diseases. Recently, numerous technological advantages have led to the evolution of DNA microarrays and microarray-based techniques, revealing the genomic modification and all transcriptional activity. Here, we show the step-by-step methods currently used in our lab to handling the oligonucleotide microarray and miRNA microarray. Moreover, we introduce the protocols of ribonucleoprotein [RNP] immunoprecipitation followed by microarray analysis (RIP-chip) which reveal the target mRNA of age-related RNA-binding proteins.

Key words

DNA microarray Gerontological study Aging miRNA RNA-binding protein RIP-chip 



The protocols above are based on the protocols establishes in Agilent Technologies (oligonucleotide microarray, and miRNA microarray) and the Laboratory of Molecular Biology and Immunology, National institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD (ribonucleoprotein immunoprecipitation followed by microarray analysis). We would like to thank Myriam Gorospe (NIA/NIH) for important technical assistance. The part of this work was supported by Grants-in-Aid for Scientific Research (C) number 24590943.


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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Kiyoshi Masuda
    • 1
  • Yuki Kuwano
    • 1
  • Kensei Nishida
    • 1
  • Kazuhito Rokutan
    • 1
  1. 1.Department of Stress ScienceInstitute of Health Biosciences, The University of Tokushima Graduate SchoolTokushimaJapan

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