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Improving Lipase Activity by Immobilization and Post-immobilization Strategies

  • Jose M. Palomo
  • Marco Filice
  • Oscar Romero
  • Jose M. Guisan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1051)

Abstract

One important parameter for the application of lipase catalysts in chemical industries is the specific activity displayed towards natural or unnatural substrates. Different strategies to enhance the lipase activity have been described. The immobilization of lipases on hydrophobic supports by interfacial adsorption at low ionic strength permitted the hyper-activation of these enzymes by fixing the open conformation of the lipase on the hydrophobic support. Improvements of activity from 1.2- up to 20-fold with respect to the initial one have been observed for lipases from different sources.

A second strategy was based on the presence of additives, in particular surfactants or ionic liquids, with hydrophobic character to enhance the activity of lipases immobilized on macroporous supports up to eightfold and even more than 100-fold in some cases for soluble lipases.

Finally, a third strategy to improve the activity in immobilized lipases was based on a site-directed chemical modification of the protein by glycosylation on the enzyme N-terminal group or on a unique reactive cysteine of the enzyme by disulfide exchange using different tailor-made disulfide activated activated polymers.

Key words

Lipase Activation Immobilization Site-directed modification Additives Polymers 

Notes

Acknowledgments

This work has been sponsored by the Spanish Ministry of Science and Innovation (AGL-2009-07526) and the CSIC by Intramural project (200980I133). The authors are grateful to CSIC for the JAE-DOC contract of M.F. and to CONICYT and Programa Bicentenario Becas-Chile for financial support of O.R.

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Jose M. Palomo
    • 1
  • Marco Filice
    • 1
  • Oscar Romero
    • 1
  • Jose M. Guisan
    • 2
  1. 1.Institute of Catalysis, CSICMadridSpain
  2. 2.Instituto de Catalisis y Petroleoquimica, CSICMadridSpain

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