Use of Microarray Analysis to Investigate EMT Gene Signatures

  • Andrew H. Sims
  • Alexey A. Larionov
  • David J. Harrison
  • Elad Katz
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1046)

Abstract

The epithelial-to-mesenchymal transition (EMT) is a widely studied program of development of cells characterized by loss of cell adhesion, repression of E-cadherin expression, and increased cell mobility. Microarrays have become a well-established technique for simultaneously measuring the expression of thousands of transcripts encoded by the genome. In this chapter, we demonstrate how microarray analysis can be used to assess the role of EMT-genes associated with a collagen invading phenotype by generating a gene expression signature and relating this to cell line and tumor datasets from published microarray studies.

Key words

Epithelium Mesenchyme Microarrays Gene expression signature Transcriptomic profiles 
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Notes

Acknowledgments

This work was supported by Breakthrough Breast Cancer and the Scottish Funding Council.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Andrew H. Sims
    • 1
  • Alexey A. Larionov
    • 1
  • David J. Harrison
    • 2
  • Elad Katz
    • 3
  1. 1.Breakthrough Breast Cancer Research UnitWestern General HospitalEdinburghUK
  2. 2.School of MedicineUniversity of St AndrewsSt AndrewsUK
  3. 3.AMS BiotechnologyAbingdonUK

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