Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein–Membrane Interactions

  • Serapion Pyrpassopoulos
  • Henry Shuman
  • E. Michael Ostap
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1046)

Abstract

Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission of forces to the plasma membrane and organelle-membranes. Despite the importance of these interactions, remarkably little is known about the mechanical properties of the bonds between membranes and proteins. In this chapter, we describe a single-molecule, optical-trapping method for the measurement of protein–membrane adhesion forces and force-dependent attachment-lifetimes.

Key words

Optical tweezers Adhesion force Single molecule Myosin-I Phosphoinositide Supported lipid bilayer Membrane 

Notes

Acknowledgment

This work was supported by a grant from the NIH (GM057247) to E.M.O.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Serapion Pyrpassopoulos
    • 1
  • Henry Shuman
    • 1
  • E. Michael Ostap
    • 1
  1. 1.Department of PhysiologyPennsylvania Muscle Institute, Perelman School of Medicine at the University of PennsylvaniaPhiladelphiaUSA

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