Imaging Gene Expression pp 101-113

Part of the Methods in Molecular Biology book series (MIMB, volume 1042)

Imaging Nascent RNA Dynamics in Dictyostelium

  • Jonathan R. Chubb
  • Michelle Stevense
  • Danielle Cannon
  • Tetsuya Muramoto
  • Adam M. Corrigan


Dictyostelium cells have great utility for live imaging of single gene transcriptional dynamics. The cells allow efficient molecular genetics, for targeting of RNA reporters and fluorescent proteins to individual, defined loci. Dictyostelium cells share many signalling, chromatin and nuclear characteristics of larger eukaryotes, yet the cells have a relatively simple scattered differentiation programme, allowing imaging of transcriptional events in the context of stochastic developmental choices. This review will detail the methods and considerations for imaging nascent RNA dynamics at single genes in living Dictyostelium cells.

Key words

Chromatin Dictyostelium Morphogen Live-cell imaging Transcriptional bursting Pulsing Stochastic gene expression Transcriptional noise Transcriptional pausing 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Jonathan R. Chubb
    • 1
  • Michelle Stevense
    • 2
  • Danielle Cannon
    • 3
  • Tetsuya Muramoto
    • 4
  • Adam M. Corrigan
    • 1
  1. 1.MRC Laboratory for Molecular Cell Biology, Department of Cell and Developmental BiologyUniversity College LondonLondonUK
  2. 2.Institut für Physiologische Chemie, Medizinische FakultätTU DresdenDresdenGermany
  3. 3.Department of Cell and Developmental Biology and MRC Laboratory for Molecular Cell BiologyUniversity College LondonLondonUK
  4. 4.Laboratory for Cell Signaling Dynamics, Quantitative Biology CenterRIKENOsakaJapan

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